CHRISTOF J. SCHWIENING Measurement of intracellular pH: a comparison between ion-sensitive microelectrodes and fluorescent dyes
نویسندگان
چکیده
Intracellular pH (pHi) has been studied extensively for over a century using a wide range of techniques. These techniques have been subject to constant improvements to the extent that useful measurements can now be made in even the smallest of cells. This chapter outlines the development of ion-sensitive microelectrodes and dyes and highlights some of the difficulties and dangers of both of these methods. It then concentrates on some recent advances in our understanding of how fluorescent pH-sensitive dyes might best be used. Finally, a tabulated comparison between the two techniques is presented.
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